{"id":3301,"date":"2019-05-17T04:52:50","date_gmt":"2019-05-17T07:52:50","guid":{"rendered":"http:\/\/www.jkscience.co.kr\/?page_id=3301"},"modified":"2019-05-18T04:44:59","modified_gmt":"2019-05-18T07:44:59","slug":"svea-column-selection","status":"publish","type":"page","link":"http:\/\/www.jkscience.co.kr\/?page_id=3301","title":{"rendered":"SVEA Column Selection"},"content":{"rendered":"\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
Phase<\/th>Characteristics<\/th>Suggested applications<\/th>Particle type<\/th>Pore size<\/th>Carbon loading<\/th>Surface area<\/th>Pore volume<\/th>End-capping<\/th>USP
\ncode<\/th>		pH range<\/th>Particle sizes<\/th>\n<\/tr>\n<\/thead>\n
C18 Gold<\/td>General first choice column. High hydrophobic retention.
\nWide range of analytes. Excellent peak shape for acids
\nand bases. High carbon content. Withstands
\ntemperatures up to 60 \u00b0C. Long life time. Widely used
\nfor the separation of various medicines, foods and
\ncosmetics.<\/td>		Cephalosporins, preservatives, sunscreens, amoxicillin, nitrofuran,
\namino acids, flavonoids, polycyclic aromatic hydrocarbons, carbamates,
\ncoal phenols, aldonic acids, tropisetron, acetylsalicylic acid, ibuprofen,
\nbase peptides, water-soluble vitamins, Chinese herbs (Eucommia),
\nchlorogenic acid, traditional Chinese medicine (astragalus), synthetic
\ndyes, queen bee acid, methylfurfural, dimethyl fumarate, synthetic
\npigments, sodium cyclohexyl sulfamate, dehydroacetic acid, natamycin,
\ntaurine, carnitine, benzopyrene, adenine, guanine, hypoxanthine,
\nnucleotides, Cefazolin, Ciprofloxacin Hydrochloride, Ethinyl Estradiol
\nImpurity, Nicotinamide, Artemether, Benzyl Alcohol, Ceftriaxone and
\nSulbactam, Cholecalciferol, D-Panthenol, Vitamin E etc<\/td>		Fully porous
\ntype B silica<\/td>		110 \u00c5<\/td>19%<\/td>300 m\u00b2\/g<\/td>0.85 ml\/g<\/td>Yes<\/td>L1<\/td>0.9-10<\/td>3.5, 5 \u00b5m<\/td>\n<\/tr>\n
C 18 Opal<\/td>Recommended for high pH applications. Proprietary
\ncoating ensures solely hydrophobic interactions. Better
\npeak shape for ionizable compounds. Withstand
\ntemperatures up to 80 \u00b0C. Can be used in aqueous
\nconditions. Has advantages over C18 Gold in multicomponent analyses. Can be used for analysis of difficult
\nexperiments, with good universality and high market
\nutilization rate.<\/td>		Macrolide antibiotics such as azithromycin US European
\nPharmacopoeia, azithromycin Chinese Pharmacopoeia system
\napplicability, roxithromycin, erythromycin, aminoglycoside, antibiotics
\nsuch as gentamicin, simvastatin, aflatoxin, natamycin, organic acids,
\nchalk, alkaloids, pigments, folic acid, benzoyl peroxide, ginsenosides,
\nirbesartan, anthocyanins, neurotransmitters, etc.<\/td>		Fully porous
\ntype B silica<\/td>		110 \u00c5<\/td>14%<\/td>300 m\u00b2\/g<\/td>0.85 ml\/g<\/td>Yes<\/td>L1<\/td>0.5-11<\/td>3.5, 5 \u00b5m<\/td>\n<\/tr>\n
C8<\/td>Similar selectivity for lipophilic compounds as C18 Gold.
\nLower retention than C18 Gold. Slightly different
\nselectivity for ionized acids and bases compared to C18
\nGold. Excellent peak shape for acids and bases. High
\nstability and longevity. Wide applicability - can be used
\nas the first choice for C8 columns.<\/td>		Water-soluble organic acid, clozapine, cefuroxime sodium, organic
\nacid, dichlorophenol and other phenolic compounds, triclosan,
\nParacetamol and Ibuprofen, etc.<\/td>		Fully porous
\ntype B silica<\/td>		110 \u00c5<\/td>11%<\/td>300 m\u00b2\/g<\/td>0.85 ml\/g<\/td>Yes<\/td>L7<\/td>1-9<\/td>3.5, 5 \u00b5m<\/td>\n<\/tr>\n
C4<\/td>Recommended for separation of large peptides and
\nproteins. Very low retention for lipophilic compounds.
\nCan also be run in HILIC-mode. The C4 column is
\noptimized for protein analysis with low adsorption and
\nhigh recovery.<\/td>		Insulin, lactoferrin, liraglutide etc. <\/td>Fully porous
\ntype B silica<\/td>		110 \u00c5<\/td>7%<\/td>300 m\u00b2\/g<\/td>0.85 ml\/g<\/td>Yes<\/td>L26<\/td>1-8<\/td>3.5, 5 \u00b5m<\/td>\n<\/tr>\n
PhHex<\/td>Orthogonal chemistry for method development. Can be
\nused in aqueous conditions. Recommended for
\nseparation of aromatics and\/or polar analytes. Long life
\ncycle.<\/td>		Pseudoephedrine, carbamazepine, verapamil hydrochloride,
\nnicardipine hydrochloride, carbamate, Anagrelide Hydrochloride<\/td>		Fully porous
\ntype B silica<\/td>		110 \u00c5<\/td>16%<\/td>300 m\u00b2\/g<\/td>0.85 ml\/g<\/td>Yes<\/td>L11<\/td>2-8<\/td>3.5, 5 \u00b5m<\/td>\n<\/tr>\n
PFP<\/td>Strong retention of protic compounds and analytes with
\nhigh dipole moments. Strong \u03c0-interaction with electron
\ndeficient aromatic rings. Recommended for very polar
\ncompounds.<\/td>		Analysis of four subtypes of tocopherol (vitamin E), matrine, isomers.<\/td>Fully porous
\ntype B silica<\/td>		110 \u00c5<\/td>11%<\/td>300 m\u00b2\/g<\/td>0.85 ml\/g<\/td>Yes<\/td>L43<\/td>2-8<\/td>3.5, 5 \u00b5m<\/td>\n<\/tr>\n
Cyano<\/td>Very polar stationary phase. Strong dipole-dipole
\ninteractions. Orthogonal phase in RPLC method
\ndevelopment. Recommended for HILIC and Normal
\nPhase. High stability and longevity.<\/td>		Benzalkonium chloride, steroids, malic acid, fumaric acid, Ondansetron<\/td>Fully porous
\ntype B silica<\/td>		110 \u00c5<\/td>7%<\/td>300 m\u00b2\/g<\/td>0.85 ml\/g<\/td>Yes<\/td>L10<\/td>2-7.5<\/td>3.5, 5 \u00b5m<\/td>\n<\/tr>\n
Core C18<\/td>Core shell technology provides high separation and low
\nback pressures. Can be used in HPLC and UPLC. Excellent
\nbase stability. Widely used in the field of rapid analysis of
\nfood, drug and cosmetics<\/td>		Flavonoids such as myricetin, quercetin, apigenin, baicalein, aflatoxin,
\nanthraquinone dyes such as alizarin, alfalfa, phenol, tea polyphenols,
\ncaffeine, gallic acid, dinitrobenzene, bisphenol A, tetracyclines,
\nethambutol hydrochloride, clenbuterol, salbutamol, ginsenosides,
\namoxicillin, clavulanate potassium, atenolol, nifedipine, nitridipine, H2
\nreceptor blocker (fumetidine, cimetidine, ranitidine), corticosteroids
\n(prednisone, cortisone, hydrocortisone, dehydrocortisol), ethinyl
\nestradiol, estrogen ketone, p-aminoacetophenone, chloroquine, oleic
\nacid, fluridic acid, hydroquinone, quinolone (2-hydroxyquinoline),
\nsulfonamides, tetracyclines (chlortetracycline, oxytetracycline,
\ndoxycycline hydrochloride), chloramphenicol , malachite green, etc.<\/td>		Coreshell
\ntype B silica<\/td>		80-100 \u00c5<\/td>6-8%<\/td>110-150 m\u00b2\/g<\/td>0.25-0.32 ml\/g<\/td>Proprietary<\/td>L1<\/td>1-10<\/td>2.6 \u00b5m<\/td>\n<\/tr>\n
Core PhHex<\/td>Orthogonal chemistry combined with core shell
\ntechnology to provide high separation and low back
\npressures. Can be used in aqueous conditions.
\nRecommended for separation of aromatics and\/or polar
\nanalytes. Can be used in HPLC and UPLC.<\/td>		Pseudoephedrine, carbamazepine<\/td>Coreshell
\ntype B silica<\/td>		80-100 \u00c5<\/td>4-5%<\/td>110-150 m\u00b2\/g<\/td>0.25-0.32 ml\/g<\/td>Proprietary<\/td>L11<\/td>1.5-9<\/td>2.6 \u00b5m<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
\"\"<\/figure>\n","protected":false},"excerpt":{"rendered":"
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