| Phase | Characteristics | Suggested applications | Particle type | Pore size | Carbon loading | Surface area | Pore volume | End-capping | USP code | pH range | Particle sizes |
|---|---|---|---|---|---|---|---|---|---|---|---|
| C18 Gold | General first choice column. High hydrophobic retention. Wide range of analytes. Excellent peak shape for acids and bases. High carbon content. Withstands temperatures up to 60 °C. Long life time. Widely used for the separation of various medicines, foods and cosmetics. | Cephalosporins, preservatives, sunscreens, amoxicillin, nitrofuran, amino acids, flavonoids, polycyclic aromatic hydrocarbons, carbamates, coal phenols, aldonic acids, tropisetron, acetylsalicylic acid, ibuprofen, base peptides, water-soluble vitamins, Chinese herbs (Eucommia), chlorogenic acid, traditional Chinese medicine (astragalus), synthetic dyes, queen bee acid, methylfurfural, dimethyl fumarate, synthetic pigments, sodium cyclohexyl sulfamate, dehydroacetic acid, natamycin, taurine, carnitine, benzopyrene, adenine, guanine, hypoxanthine, nucleotides, Cefazolin, Ciprofloxacin Hydrochloride, Ethinyl Estradiol Impurity, Nicotinamide, Artemether, Benzyl Alcohol, Ceftriaxone and Sulbactam, Cholecalciferol, D-Panthenol, Vitamin E etc | Fully porous type B silica | 110 Å | 19% | 300 m²/g | 0.85 ml/g | Yes | L1 | 0.9-10 | 3.5, 5 µm |
| C 18 Opal | Recommended for high pH applications. Proprietary coating ensures solely hydrophobic interactions. Better peak shape for ionizable compounds. Withstand temperatures up to 80 °C. Can be used in aqueous conditions. Has advantages over C18 Gold in multicomponent analyses. Can be used for analysis of difficult experiments, with good universality and high market utilization rate. | Macrolide antibiotics such as azithromycin US European Pharmacopoeia, azithromycin Chinese Pharmacopoeia system applicability, roxithromycin, erythromycin, aminoglycoside, antibiotics such as gentamicin, simvastatin, aflatoxin, natamycin, organic acids, chalk, alkaloids, pigments, folic acid, benzoyl peroxide, ginsenosides, irbesartan, anthocyanins, neurotransmitters, etc. | Fully porous type B silica | 110 Å | 14% | 300 m²/g | 0.85 ml/g | Yes | L1 | 0.5-11 | 3.5, 5 µm |
| C8 | Similar selectivity for lipophilic compounds as C18 Gold. Lower retention than C18 Gold. Slightly different selectivity for ionized acids and bases compared to C18 Gold. Excellent peak shape for acids and bases. High stability and longevity. Wide applicability - can be used as the first choice for C8 columns. | Water-soluble organic acid, clozapine, cefuroxime sodium, organic acid, dichlorophenol and other phenolic compounds, triclosan, Paracetamol and Ibuprofen, etc. | Fully porous type B silica | 110 Å | 11% | 300 m²/g | 0.85 ml/g | Yes | L7 | 1-9 | 3.5, 5 µm |
| C4 | Recommended for separation of large peptides and proteins. Very low retention for lipophilic compounds. Can also be run in HILIC-mode. The C4 column is optimized for protein analysis with low adsorption and high recovery. | Insulin, lactoferrin, liraglutide etc. | Fully porous type B silica | 110 Å | 7% | 300 m²/g | 0.85 ml/g | Yes | L26 | 1-8 | 3.5, 5 µm |
| PhHex | Orthogonal chemistry for method development. Can be used in aqueous conditions. Recommended for separation of aromatics and/or polar analytes. Long life cycle. | Pseudoephedrine, carbamazepine, verapamil hydrochloride, nicardipine hydrochloride, carbamate, Anagrelide Hydrochloride | Fully porous type B silica | 110 Å | 16% | 300 m²/g | 0.85 ml/g | Yes | L11 | 2-8 | 3.5, 5 µm |
| PFP | Strong retention of protic compounds and analytes with high dipole moments. Strong π-interaction with electron deficient aromatic rings. Recommended for very polar compounds. | Analysis of four subtypes of tocopherol (vitamin E), matrine, isomers. | Fully porous type B silica | 110 Å | 11% | 300 m²/g | 0.85 ml/g | Yes | L43 | 2-8 | 3.5, 5 µm |
| Cyano | Very polar stationary phase. Strong dipole-dipole interactions. Orthogonal phase in RPLC method development. Recommended for HILIC and Normal Phase. High stability and longevity. | Benzalkonium chloride, steroids, malic acid, fumaric acid, Ondansetron | Fully porous type B silica | 110 Å | 7% | 300 m²/g | 0.85 ml/g | Yes | L10 | 2-7.5 | 3.5, 5 µm |
| Core C18 | Core shell technology provides high separation and low back pressures. Can be used in HPLC and UPLC. Excellent base stability. Widely used in the field of rapid analysis of food, drug and cosmetics | Flavonoids such as myricetin, quercetin, apigenin, baicalein, aflatoxin, anthraquinone dyes such as alizarin, alfalfa, phenol, tea polyphenols, caffeine, gallic acid, dinitrobenzene, bisphenol A, tetracyclines, ethambutol hydrochloride, clenbuterol, salbutamol, ginsenosides, amoxicillin, clavulanate potassium, atenolol, nifedipine, nitridipine, H2 receptor blocker (fumetidine, cimetidine, ranitidine), corticosteroids (prednisone, cortisone, hydrocortisone, dehydrocortisol), ethinyl estradiol, estrogen ketone, p-aminoacetophenone, chloroquine, oleic acid, fluridic acid, hydroquinone, quinolone (2-hydroxyquinoline), sulfonamides, tetracyclines (chlortetracycline, oxytetracycline, doxycycline hydrochloride), chloramphenicol , malachite green, etc. | Coreshell type B silica | 80-100 Å | 6-8% | 110-150 m²/g | 0.25-0.32 ml/g | Proprietary | L1 | 1-10 | 2.6 µm |
| Core PhHex | Orthogonal chemistry combined with core shell technology to provide high separation and low back pressures. Can be used in aqueous conditions. Recommended for separation of aromatics and/or polar analytes. Can be used in HPLC and UPLC. | Pseudoephedrine, carbamazepine | Coreshell type B silica | 80-100 Å | 4-5% | 110-150 m²/g | 0.25-0.32 ml/g | Proprietary | L11 | 1.5-9 | 2.6 µm |
